Fig. 6: The Ribo-On system enables spatiotemporal turn-on of endogenous gene expression. | Communications Biology

Fig. 6: The Ribo-On system enables spatiotemporal turn-on of endogenous gene expression.

From: Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

Fig. 6: The Ribo-On system enables spatiotemporal turn-on of endogenous gene expression.The alternative text for this image may have been generated using AI.

a Schematic representing how ribozyme/Cre-loxp systems conditionally turn on gene expression. b The expression of ARGN-1::GFP in the epidermis and the mitochondrial morphology in one-day-old adult worms under different genetic backgrounds. Cre expression was spatially controlled by tissue-specific promoters or temporally induced by heat-shock promoter, respectively. Scale bars: 5 μm. Arrows: enlarged mitochondria. c Quantification of ARGN-1::GFP expression in the epidermis under different conditions, as shown in (b). Each dot represents a single worm, n ≥ 25. The exact number of animals is provided as source data. All data are presented as mean ± s.e.m. *p < 0.05. ****p < 0.0001 (one-way ANOVA with the Tukey correction). d Quantifications of the proportion of animals containing normal, intermediate defective or severe defective morphology of mitochondrial in the epidermis under different conditions as shown in (b). The number of animals quantified was shown above each column. Source data are provided as a Source Data file.

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