Fig. 3: Global ASIC1a deletion affects body temperature and thyrotropin-releasing hormone mRNA levels in the hypothalamus.

a, b Mice were kept under a 12 h light:12 h dark (LD) cycle. Body temperature and locomotor activity of mice was measured with an implanted sensor. a Core body temperature rhythm for 24 h under LD cycle, n = 10 WT and 11 ASIC1a^−/− mice. b Daily profile of locomotor activity in LD cycle. Activity counts are expressed as counts/s; n = 10 WT and 11 ASIC1a^−/− mice. c-d RT-qPCR analysis of the daily profile of Trh expression in hypothalamus of WT (c) and ASIC1a^−/− mice (d). Results for each mouse are presented as relative expression normalized to the mean at ZT0; n = 6–7 animals per condition. e Trh mRNA expression in the PVH at ZT1 and ZT13; normalization to the mean WT value at ZT1; tissue from n = 5–7 animals per condition. Statistical analysis was done on absolute values (a, b) or log2-transformed data in c–e. **p < 0.01; ***p < 0.001, different between genotypes, by two-way ANOVA, repeated measures, Sidak post hoc test (a, b). ^#p < 0.05; ^##p < 0.01; ^####p < 0.0001; comparison between time points; ***p < 0.0001, between genotypes (c–e); one-way ANOVA and Tukey post hoc test (c, d) and two-way ANOVA and Tukey post hoc test (e). Error bars indicate SEM.