Fig. 6: ASIC1a regulates circadian rhythm behaviors.

a Representative actograms of wheel-running activity profile of a WT and ASIC1a^−/− mouse under 12 h light ∶ 12 h dark (LD) cycle or constant dark (DD, lower half) are shown. Periods of darkness are shown by dark shading. Animals were habituated as previously described⁶⁰. b The 7-day average activity of WT and ASIC1a^−/− mice under LD conditions (corresponding to days 2–8) was quantified, n = 11 WT and 11 ASIC1a^−/− mice. *p < 0.05; **p < 0.01; ***p < 0.001, the difference between genotypes, two-way ANOVA repeated measures and Sidak post hoc test. c WT and ASIC1a^−/− mice were transferred to DD cycle and locomotor activity recordings from days 10 to 16 in free-running conditions were used to assess their internal period length with the χ²-periodogram analysis, n = 11 WT and 11 ASIC1a^−/− mice. ***p < 0.001, comparison between WT and ASIC1a^−/− mice, t test. d Phase shifts after light pulses according to the Aschoff type 2 protocol. For each light pulse (ZT14 and ZT22), animals were entrained to an LD cycle for 2 weeks before being released into constant darkness for 10 d. The pulse was administered on the first night after the end of LD, and phase shifts were calculated, n = 11 WT and 11 ASIC1a^−/− mice (two-way ANOVA). Statistical analysis was done on non-transformed data. Error bars indicate SEM.