Fig. 2: IEM observation revealed that extracellular Pgk1 and endogenous membrane protein Eno2 were located closely on the cell surface of neural cells. | Communications Biology

Fig. 2: IEM observation revealed that extracellular Pgk1 and endogenous membrane protein Eno2 were located closely on the cell surface of neural cells.

From: Extracellular Pgk1 interacts neural membrane protein enolase-2 to improve the neurite outgrowth of motor neurons

Fig. 2

a–h NSC34 neural cells were incubated in the medium containing either EGFP-Flag (a–d), which served as a negative control, or recombinant Pgk1-Flag (e–h). Double immunogold labeling was performed on the ultrathin sections of cells and examined at low magnification (X 8000) (a, e). b–d, f–h were amplified at high magnification (X 80,000) from the rectangular areas indicated within (a, e), respectively. Ultrathin sections were reacted simultaneously with mouse anti-Eno2 antibody and rabbit anti-Flag antibody, followed by 12-nm gold-conjugated anti-mouse IgG antibody (black arrowheads) and 18-nm gold-conjugated anti-rabbit IgG antibody (white arrowheads). Scale bar indicates 2 μm (a, e) and 100 nm (b–d, f–h). Data were averaged from three independent experiments.

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