Fig. 4: cf-mRNAs are enriched and protected in EVs.
From: Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles
a Expression of protein markers (CD9, ApoA1, Ago2, and ApoB) using immunoprecipitation across plasma fractions. Ladder was cropped from full gel to show size reference. b RT-qPCR analysis of selected genes using supernatants eluted from CD9, ApoB, or IgG immunocaptures from FR14 and FR58. P-value is derived from the Tukey’s test (ns = not significant, P > 0.05; *P < 0.05, **P < 0.01, and ****P < 0.0001) with three technical replicates of FR14 and FR58 of plasma pooled from three healthy individuals. c A box plot of negative raw cycle threshold (- raw ct) of individual genes with RNase and/or detergent treatment using qRT-PCR. RNA isolated from combined FR14 and FR58 using three healthy individuals and three technical replicates of control RNA were treated with RNase and/or detergent. Data are analyzed using RStudio and reported as means ± SD of three independent samples.
