Fig. 8: ABL1 deficiency in C. elegans increases lifespan in an ATG18-dependent manner.
From: The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension
a For lifespan assessments, eggs were isolated by hypochlorite treatment and grown on NGM/OP50 plates until they reached the L4 larval stage. L4 nematodes were then transferred to NGM/OP50/FUdR plates to sterilise the nematodes, and surviving nematodes were counted every 2–3 days. The resulting lifespan curves are shown (statistical OASIS analysis, Supplementary Table 1). b Likewise, lifespan assessments were conducted while depleting the c-MYC homologue MML-1 in N2 wild type (mml-1 (RNAi)) or the ULK homologue UNC-51 in the abl-1(ok171) strain (abl-1(ok171); unc-51 (RNAi)) by RNA interference. Lifespan curves are shown and statistical OASIS analysis is displayed in Supplementary Table 2). c A predicted model for the regulation of WIPI1 gene expression by the ABL/MYC axis and its impact on autophagy and lifespan in C. elegans. Created with BioRender.com.
