Fig. 5: Phylogenetic tree and expression pattern of candidate families for anthraqunine tailoring.

a Phylogenetic analysis of A-type (left) and non-A-type (right) CYP450 families. The red and blue branches indicate the sequences from R. tanguticum and Arabidopsis thaliana, respectively. The red background color of each gene ID also indicates sequences from R. tanguticum. The round rectangle beside each gene ID represents the gene’s module color from the WGCNA analysis. The outermost circle indicates the CYP450 gene family. The outermost circle of non-A-type CYP450 phylogenic tree indicates the CYP450 gene family clan. b The expression pattern of all A-type CYP450 members. The colored bar indicates the range of expression levels for genes. The colors of the rounded rectangles represent the different expression levels as illustrated by the legend. c Phylogenetic tree of BGLs based on the protein sequence alignments from R. tanguticum and Arabidopsis. d Expression analysis of BGL genes in eight different tissues. The dot sizes and colors represent the different expression levels as illustrated by the legend.