Fig. 4: Structural analysis of hAMPs.
From: Molecular hybridization strategy for tuning bioactive peptide function
Circular dichroism (CD) spectra of the parent peptides and the hAMPs designed in this study in (a) water, (b) PBS (pH 7.4), (c) SDS (20 mmol L−1), (d) POPC (10 mmol L−1), (e) POPC:POPG (3:1, mol:mol - 10 mmol L−1), (f) POPC:DOPE (3:1, mol:mol - 10 mmol L−1), and (g) TFE/Water (3:2, v-v). CD spectra were recorded after four accumulations at 20 °C, using a 1 mm path length quartz cell, between 195 and 260 nm at 100 nm min−1, with a band width of 0.5 nm. All assays were performed with peptides at 50 μmol L−1. h Helical fractions (fH) values were calculated based on the Lifson-Roig model for each of the conditions analyzed (a–g).
