Fig. 2: Steady-state ATPase activity of wild-type CCT/TRiC, the single mutants, T394P and R510H, in subunit CCT2 and the corresponding double mutant at different concentrations of ATP. | Communications Biology

Fig. 2: Steady-state ATPase activity of wild-type CCT/TRiC, the single mutants, T394P and R510H, in subunit CCT2 and the corresponding double mutant at different concentrations of ATP.

From: Reduced ADP off-rate by the yeast CCT2 double mutation T394P/R510H which causes Leber congenital amaurosis in humans

Fig. 2

Initial velocities of ATP hydrolysis by wild-type CCT/TRiC and the different mutants were measured at different concentrations of ATP using a final oligomer concentration of CCT/TRiC of 20 nM. The data for wild-type CCT/TRiC (a) and the single mutants (b) were fitted to Eq. 1 for two allosteric transitions. See Materials and Methods for more details. Error bars represent standard errors.

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