Fig. 3: Validation of cochlin gene and protein expression in FDM and LIM models. | Communications Biology

Fig. 3: Validation of cochlin gene and protein expression in FDM and LIM models.

From: Targeting the cochlin/SFRP1/CaMKII axis in the ocular posterior pole prevents the progression of nonpathologic myopia

Fig. 3

The expression of the Coch gene in the FDM group during the first 3 w of myopia induction is shown in a and was compared with that in the normal control group using two-way ANOVA (n = 5–8). Representative blot of cochlin protein expression during the initial 3-w induction is shown in b. The blots were quantified in c and compared using two-way ANOVA (n = 5–9). The differences in cochlin protein levels between treated and untreated eyes were negatively correlated with the refraction differences between the 2 eyes during the 1st (d), 2nd (f), and 3rd (h) w of FDM modeling. The cochlin expression differences between treated and untreated eyes were positively correlated with the AL differences between the 2 eyes during the 1st (e), 2nd (g), and 3rd (i) w of FDM modeling. Coch gene expression levels in LIM and FDM models at 6 w post induction were shown in j and compared with those of the normal controls using one-way ANOVA (n = 7–14). Representative blots of the cochlin protein in LIM and FDM models at 6 w after myopic induction (k). The blots were quantified in l and compared using one-way ANOVA (n = 8). Representative pictures of cochlin immunohistochemistry staining in the normal control, LIM, and FDM groups at 6 w post myopia induction (m). Scale bar = 20 μm. The mean staining intensity was quantified in n and compared using one-way ANOVA (n = 9). All data represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. NOR normal control, FDM form-deprived myopia, LIM lens-induced myopia, AL axial length, w- week, AU arbitrary units.

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