Fig. 3: The experimental 2D IR spectra in the amide I stretch region reveal multiple bands corresponding to different secondary structures.
From: Crowding alters F-actin secondary structure and hydration
Spectra measured at pump-probe delay times (t2) of 0.15 ps (top) and 2.0 ps (bottom) are shown as examples. Three columns from left to right correspond to the filament, weakly bundled network, and strongly bundled network, respectively, as indicated in the titles. The X- and Y-axes represent the pump and probe frequencies, respectively. The spectra are normalized to the maxima of the peak intensity for comparison among the different samples. The solid black lines represent the diagonals of the spectra. The dashed lines represent the guidance of the frequency positions (1630, 1650, and 1675 cm−1). Arrows indicate the rough positions of the cross peaks. The contour bars to the right of the axes display the current color map and indicate the mapping of data values into the color map. The background of the spectra (peak amplitude below 0.2) is removed for clarity.
