Fig. 5: EVX1AS analogues are functionally convergent among species.
From: Functional evolutionary convergence of long noncoding RNAs involved in embryonic development
a Relative RNA expression of human EVX1AS lncRNA and EVX1 upon human EVX1AS overexpression in SHSY5Y cells. b Relative RNA expression of gecko EVX1AS-like lncRNA and human EVX1 upon pp-EVX1AS-like overexpression in SHSY5Y cells. Human RPLP0 was used as housekeeping control and normalizations were done to the highest value in each experiment. c Immunohistochemistry of the chicken embryonic midbrain on coronal section showing the successful transfection of the lncRNA plasmid. Transfected cells are shown in red due to their ectopic expression of red fluorescent protein RFP. LHX5 and MEIS2 patterns of expression (in green) indicate the mesencephalic region that was actually transfected. DAPI counterstain in blue. d Relative RNA expression of human EVX1AS lncRNA and chicken Evx1 upon human EVX1AS overexpression in chicken mesencephalon. Scale bars represent 1 mm, 500 micras and 100 micras from top to bottom. e Relative RNA expression of gecko EVX1AS-like lncRNA and chicken Evx1 upon pp-EVX1AS-like overexpression in chicken mesencephalon. Chicken Rplp7 was used as housekeeping control. Data represents the mean and standard error of 3–6 independent experiments. (+P < 0.1, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 according to one-tailed Student’s t test). HS1 and HS2: sgRNAs targeting human EVX1 TSS1 and TSS2, respectively. GG1 and GG2: sgRNAs targeting chicken Evx1 TSS1 and TSS2, respectively.
