Fig. 1: Temperature preference of the three Drosophila species.

Boxplot illustrating the preferred temperatures of males and females of a D. ezoana, b D. novamexicana and c D. virilis when reared at 20 °C (left), 23 °C (middle) and 25 °C (right). Boxplot whiskers indicate ± 1.5 interquartile range limits. Dots in each boxplot indicate individual temperature data points (n = 50 biologically independent samples). Significance (P < 0.05) was tested in an unpaired t-test (comparison between sexes of each species) and a one-way ANOVA followed by SNK multiple comparison post hoc tests (comparison across species).