Fig. 4: Controlling nutrient starvation and cell viability in the Griddient. | Communications Biology

Fig. 4: Controlling nutrient starvation and cell viability in the Griddient.

From: Griddient: a microfluidic array to generate reconfigurable gradients on-demand for spatial biology applications

Fig. 4: Controlling nutrient starvation and cell viability in the Griddient.

HCT-116 cells were stained with CellTracker™ Green CMFDA and seed at 30 million cells/mL. a–c Fluorescence images of the Griddient with different media/PBS configuration. Media was replaced by PBS as represented in the left panels. After 24 h cell viability was evaluated staining dead cells with PI. Images show the overlay between total cells and dead cells. Graph shows the fluorescence intensity profile of necrotic cells across Griddient n = 3. d Center wells of Griddient were treated with puromycin 5 µg/mL. After 24 h, cell viability was evaluated staining death cells with PI. Images show the overlay of viable and dead cells. Graph shows the fluorescence intensity profile of necrotic cells across the Griddient platform.

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