Fig. 5: Generation of biopsy and grafts in Griddient platform.

a schematic representation of sample biopsy from one port in the Griddient platform. b HCT-116 cells were stained with CellTracker™ Deep Red Dye and seeded in collagen at 30 million cells/well. After gel polymerization, a biopsy was taken from one of the ports. Fluorescence images of the gel before and after biopsy. c–e The biopsy sample was stained with calcein (green, viable cells) and PI (red, dead cells). Cell images before (c) and after (d) biopsy digestion. e Quantification of cell viability in the biopsy.). Results are plotted as mean ± SEM; n = 4. HCT-116 cells plated in a 2D culture plate were used as a control. f First, a biopsy was performed in the gel. The cavity was filled with a collagen solution with higher cell density. Cells were stained with Cell Tracker (green) and ImageiT Hypoxia Reagent (red).