Fig. 1: Concept of time-dependent cell-state selection, TDCSS.

a Model depicting the transition of cellular states during the process of cell fate determination. b Variation in the timing of fate determination among cells. c Comparison of two cell recovery methods during the process of cell fate determination. The conventional strategy involves collecting cells in a “snapshot” manner (black dashed rectangle), while the “time-dependent” method involves collecting cells at each cell’s individual transition timing (green dashed circle). d Single-cell transcriptomes obtained through snapshot cell recovery methods and inferred trajectories of fate determination. e TDCSS methodology combines longitudinal and comprehensive analysis. f Single-cell transcriptomes obtained through time-dependent cell recovery methods and deduced trajectories of fate determination. g Schematic representation of the TDCSS technique.