Fig. 1: Synthesis, assembly, and function of phycobiliprotein and both photosystems during recovery from nitrogen chlorosis. | Communications Biology

Fig. 1: Synthesis, assembly, and function of phycobiliprotein and both photosystems during recovery from nitrogen chlorosis.

From: Identification of a c-type heme oxygenase and its function during acclimation of cyanobacteria to nitrogen fluctuations

Fig. 1

a Time-course synthesis profile of phycobiliprotein (PBP) and both photosystems (PSs) after the nitrate is added to chlorotic cells. Total protein corresponding to 3 × 107 cells was loaded onto each lane and NdhI was used as a sample loading control. b Quantification of PBP (CpcA and ApcA, n = 4), PSs (D1, PsbO, PsaB, and PsaD, n = 3) and NDH-1 (NdhI, n = 3) synthesis levels using ImageJ. c, d Time-course assembly profiles of phycobilisome (PBS) isolated by sucrose density gradient centrifugation (c) and PSs isolated by BN-PAGE (d) after the nitrated is added to chlorotic cells. e Time-course function profiles of PBS and PSs after the nitrate is added to chlorotic cells. Red lines (circle): PBS (n = 6); blue lines (triangle up): PSII (n = 12); green lines (square): PSI (n = 12). f A model schematically represents the temporal sequence of synthesis, assembly and function of PBP and PSs during recovery from nitrogen chlorosis.

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