Fig. 2: Ssr1698 is of great importance for synthesis, assembly, and function of phycobiliprotein during recovery from nitrogen chlorosis. | Communications Biology

Fig. 2: Ssr1698 is of great importance for synthesis, assembly, and function of phycobiliprotein during recovery from nitrogen chlorosis.

From: Identification of a c-type heme oxygenase and its function during acclimation of cyanobacteria to nitrogen fluctuations

Fig. 2

a A comparative transcriptomic analysis was conducted after the nitrate was added to chlorotic cells for 4 h or not, and a top one upregulated unknown gene ssr1698 (see arrow) in our transcriptomic data was selected as a candidate for synthesis, assembly, and function of phycobiliprotein after the nitrogen becomes available. b Construction of the plasmid used to generate the ssr1698-deletion mutant (Δssr1698). c Western analysis of Ssr1698 from the total protein of the WT (including indicated serial dilutions) and Δssr1698 strains. Total protein corresponding to 1 µg chlorophyll a was loaded onto each lane, and NdhI was detected as a loading control. d–f Deletion of ssr1698 impairs the synthesis (d), assembly (e) and function (f) of PBP after the nitrate is added to chlorotic cells for 12 h. Error bars denote the standard deviations of eight independent measurements (n = 8); ***Represents P < 0.0001 (P = 1.5 × 10−7).

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