Fig. 1: Lip-SMap on extracted proteomes of photosynthetic and chemoautotrophic bacteria.
From: Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation
a Workflow of Lip-SMap based on Piazza et al.5. Blue ticks represent digestion by trypsin/LysC, and red ticks represent digestion by proteinase K. Peptides cut by proteinase K are not detected, and differences in such digestion cause differentially abundant peptides. b Peptide coverage of Calvin cycle enzymes. LiP-SMap-detected peptides from Calvin cycle enzymes of Synechocystis and Cupriavidus are colored in light gray. Regions of proteins with no peptide coverage are colored in dark gray. Significantly altered peptides when extracts were treated with GAP and acetyl-CoA are colored in red or blue, respectively. Peptides that are significantly altered for both metabolites are colored in purple. For high-concentration tests, 5 mM GAP or 10 mM acetyl-CoA was added. For low-concentration tests, 0.5 mM GAP or 1 mM acetyl-CoA was added.
