Table 1 Effect of LiP-SMap metabolites on F/SBPase activity and thermal stability in vitro.
From: Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation
Effector (mM) | LiP-SMap interaction | Kinetics change | Tm change |
|---|---|---|---|
syn-F/SBPase | |||
Acetyl-CoA (2) | Yes | Not significant | Not significant |
AMP (0.25) | Noa | Inhibit | Increase |
Citrate (5) | Yes | Inhibit | Decrease |
GAP, +DTT (0.5) | Not tested | Stimulate | Increase |
GAP, −DTT (0.5) | Yes | Inhibit | Not significant |
NADPH (3) | Yes | Inhibit | Not significant |
G6P (2) | No | Not significant | Not tested |
cn-F/SBPase | |||
AMP (0.25) | No | Inhibit | Increase |
GAP, +DTT (0.5) | Not tested | Stimulate | Not significant |
GAP, −DTT (0.5) | Yes | Not tested | Not significant |
NADPH (3) | Yes | Inhibit | Increase |
G6P (2) | Yes | Stimulate | Not significant |
