Fig. 2: Monoclonal antibodies and biparatopic antibodies against TNFR2. | Communications Biology

Fig. 2: Monoclonal antibodies and biparatopic antibodies against TNFR2.

From: Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by reducing signaling activity through epitope selection

Fig. 2

a Topographical epitope mapping of the anti-TNFR2 mAbs by mutual competition analysis. Strengths of competition are shown as percentages in each box. b Epitopes of the selected monoclonal antibodies identified using the mutants. Cyan, TNFR2; white, TNFα (PDB entry: 3ALQ). c Schematic presentation of the epitopes (red circles). Cysteine-rich domains (CRDs) are separated by lines. d Produced BpAbs. Fab ‘N’ (row) and ‘C’ (column) were fused with IntN or IntC-Fc, respectively, and were subjected to intein-mediated protein trans-splicing to produce a BpAb38. e Agonistic (upper) and antagonistic (lower) activity of conventional and biparatopic antibodies, monitored using a reporter cell line. Values are shown with the standard deviation of five independent experiments standardized by the reporter activity of 0 and 50 ng/mL TNFα.

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