Fig. 3: Size of the immunocomplexes formed by conventional and biparatopic antibodies. | Communications Biology

Fig. 3: Size of the immunocomplexes formed by conventional and biparatopic antibodies.

From: Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by reducing signaling activity through epitope selection

Fig. 3

The size was analyzed by SEC-MALS (a, e, f) or mass photometry (b–d). a Selected chromatograms of SEC-MALS. Relative refractive index and molar mass are represented by thin and bold lines, respectively. b–d Relative frequency of the observed particles with their respective mass in mass photometry. Bp109-96 (b), Bp96-92 (c), or Bp109-92 (d) was mixed with 1 eq. or 8 eq. (only for Bp109-92) of sTNFR2. Peak molecular weight (Mp) (e) or weight-averaged molecular weight (Mw) (f) of the total fractions of immunocomplexes (eluted earlier than 14.8 mL). Chromatograms of underlined antibodies are shown in (a). Colored bands represent the estimated size of the immunocomplex with the expected structures in the right column.

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