Table 1 Cryo-EM data collection and image processing of Bp109-92–TNFR2-MBP complex.
Bp109-92–TNFR2-MBP complex (EMDB-34871) (PDB 8HLB) | |
|---|---|
Data collection and processing | |
Magnification | 60,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 60 |
Defocus range (μm) | −0.5 to −2.0 |
Pixel size (Å) | 1.088 |
Symmetry imposed | C1 |
Micrographs used (no.) | 4,160 |
Initial particle images (no.) | 2,218,071 |
Final particle images (no.) | 100,391 |
Map resolution (Å) | 3.63 |
FSC threshold | 0.143 |
Refinement | |
Initial model used (PDB code) | 3ALQ |
Model resolution (Å) | 3.5/3.6/4.0 |
FSC threshold | 0/0.143/0.5 |
Model vs. Data CC (mask) | 0.75 |
(volume) | 0.73 |
Model composition | |
Non-hydrogen atoms | 7464 |
Protein residues | 977 |
Ligands | 0 |
R.m.s. deviations | |
Bond lengths (Å) | 0.006 |
Bond angles (°) | 0.761 |
Validation | |
MolProbity score | 2.05 |
Clashscore | 10.35 |
Poor rotamers (%) | 0 |
Ramachandran plot | |
Favored (%) | 91.21 |
Allowed (%) | 8.38 |
Outliers (%) | 0.41 |
