Fig. 2: BrafV600E expression in the mouse intestinal epithelium alters intestinal homeostasis and affects absorptive and secretory cell lines.
a GSEA showing changes in differentiation gene signatures 3 days and 6 months following tamoxifen induction. b GSEA showing changes in enterocyte gene signatures 6 months following tamoxifen induction. NES normalized enrichment score. c Histological images of small intestinal tissue from WT control mice and mice with the BrafV600E mutation (BVE) 6 months following tamoxifen induction stained with alkaline phosphate (Alpl) to assess enterocyte cells. Bar size = 100 μm. d TEM images of WT and Braf-mutant intestinal tissues showing altered microvilli in mutant mice. scale bar = 2 μm. e Quantification of Goblet and ChgA+ enteroendocrine cells from the indicated mice. Each dot represents a single mouse, and lines represent mean ± SEM. Data were analyzed by unpaired two-tailed t test (n = 3 per group). *P ≤ 0.05, **P ≤ 0.001. f rt-qPCR analysis of the expression of the intestinal biomarkers Chromogranin A, (ChgA, EECs) 6 months after induction of BrafV600E. Data are plotted as mean ± SD. Dots represent single replicates. Data were analyzed by unpaired two-tailed t test (n = 4 per group). *P ≤ 0.05.
