Fig. 5: The chromatin landscape of M. jannaschii.
From: DNA-bridging by an archaeal histone variant via a unique tetramerisation interface
a MNase-resistant DNA species from M. jannaschii chromatin span a wide size range and exhibit 30 bp periodicity consistent with variably-sized hypernucleosome organisation. MNase-seq fragment size versus relative frequency is plotted for deproteinised genomic DNA and bulk chromatin from cells in log growth phase treated with 3 or 8 units/ml MNase. b Chromatin particles are positioned with respect to transcription start sites and level of transcription. Normalised chromatin particle mid-point frequencies (y axis) from logarithmic growth phase cells were plotted relative to transcription start sites (10 bp bin size) across increasing chromatin particle sizes (15 bp bins). To ameliorate MNase cleavage bias, the signal for deproteinised genomic DNA was subtracted72. Values >0 are coloured yellow. Transcript level estimates were obtained from previously published RNA-seq data28.
