Fig. 1: Identification of necroptosis inhibitors using a library of 1965 small-compounds. | Communications Biology

Fig. 1: Identification of necroptosis inhibitors using a library of 1965 small-compounds.

From: Repurposing of Ibrutinib and Quizartinib as potent inhibitors of necroptosis

Fig. 1: Identification of necroptosis inhibitors using a library of 1965 small-compounds.The alternative text for this image may have been generated using AI.

a Schematic overview of compound screen workflow. b L929 cells were pretreated with individual compound for 1 h following treatment with TNF (10 ng/ml), Smac mimetic (SM-164) (0.1 μM) and pan-caspase inhibitor z-VAD (10 μM) (TSZ) to induce necroptosis. Cell viabilities were determined with Cell Counting Kit-8 method and normalized to control (DMSO + TSZ). The concentration of individual compound is 15 μM. NEC-1 (20 μM) was chose as positive control. The red line indicated fold change compared to the control is 4. The screen data for each compound was singlicate. c Classified necroptosis inhibitors into known or novel inhibitors according to whether compounds had been reported to involved in necroptosis. d Classified necroptosis inhibitors into different groups according to their properties.

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