Fig. 2: Qualitative and quantitative in vivo imaging of PDAC tumor in PDX mouse using SLAM microscopy. | Communications Biology

Fig. 2: Qualitative and quantitative in vivo imaging of PDAC tumor in PDX mouse using SLAM microscopy.

From: In vivo label-free optical signatures of chemotherapy response in human pancreatic ductal adenocarcinoma patient-derived xenografts

Fig. 2: Qualitative and quantitative in vivo imaging of PDAC tumor in PDX mouse using SLAM microscopy.The alt text for this image may have been generated using AI.

Example data is from a tumor responsive to FIRINOX (group 1) after 1 week of treatment. a Skin flap outlined in blue and approximate tumor in the yellow box. Inset is an H&E-stained histology image of the corresponding skin flap. b Large-area mosaic SLAM image of the skin flap in (a) with approximate tumor area located in yellow box. c Example FOV within the tumor from the red box in (b). The tumor margin is indicated with a red dashed line. The light blue dashed circles show clusters of cancer cells. d H&E-stained histology image of (c). e SHG, (f) THG, (g) 2PF, and (h) 3PF individual SLAM channels from (c). i Zoomed in the area from the central region of (c), with visible EVs indicated by small bright spots in the THG channel and annotated with white arrows. j EV segmentation mask, which enables quantification of EV density, EV ORR, and a fraction of NAD(P)H-rich EVs. k Example quantification of image channels and ORR from (c), with each point representing a 100×100 μm2 region within the tumor images.

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