Fig. 5: Increased Na+ currents in Slack−/− hippocampal neurons.
a–e Voltage-clamp recordings of whole-cell currents from 14 div hippocampal neurons. a Representative recordings from Slack+/+ and Slack−/− neurons responding to depolarizing voltage steps (from −60 to +80 mV in 20 mV increments) before (left), after perfusion with TTX (middle) and following digital subtraction (right). b (Magnification from a) Depolarization-induced, TTX-sensitive transient inward currents. c Compared to Slack+/+ (n = 20 from six preparations), TTX-sensitive inward current amplitudes in Slack−/− (n = 17 from 7 preparations) neurons were significantly (p < 0.0168) increased (two-way ANOVA, F1,280 = 5.791). d TTX-sensitive steady-state outward currents were significantly (two-way ANOVA, F1,312 = 5.122, p = 0.0243) higher in Slack+/+ (n = 24) compared to Slack−/− (n = 17). e (Magnification of red section from d) TTX-sensitive steady-state currents at voltage steps from −40 to +20 mV indicated an enlarged inward-directed current component in Slack−/− probably representing INaP. Data represented as mean ± SEM with p < 0.05. For detailed statistics also consult Supplementary Data 1.
