Fig. 5: Rare antibody phage isolation and discrimination (RAPID) biopanning of Gαq. | Communications Biology

Fig. 5: Rare antibody phage isolation and discrimination (RAPID) biopanning of Gαq.

From: Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets

Fig. 5

a RAPID flow cytometry of Rounds 1–6. Round 5 shows the highest normalized median fluorescent intensity (NMFI) of 2.36. b Overlay of RAPID flow cytometry of round 4–6 and a no phage control. An increase in standard deviation (SD) of the fluorescent distribution is observed in round 5. c Biolayer interferometry antibody screen (BIAS) koff of candidate hits. Blue data points represent identical values, while red data points also represent identical values.

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