Fig. 6: Rare antibody phage isolation and discrimination (RAPID) biopanning of cyclic STAT3 decoy (CS3D). | Communications Biology

Fig. 6: Rare antibody phage isolation and discrimination (RAPID) biopanning of cyclic STAT3 decoy (CS3D).

From: Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets

Fig. 6

a RAPID flow cytometry of biopanning campaign rounds 1–5. No significant increase in normalized median fluorescent intensity (NMFI) is observed. b Zoom in of (a) populations where FITC-A > 103. Maximum NMFI is exhibited in round 4. c Overlay of RAPID flow cytometry of round 4 (red, unfilled) and the original library control (gray, filled), d Biolayer interferometry antibody screen (BIAS) koffs of the top 4% and the bottom 96% of round 4 randomly chosen clones. The top ~4% sorted population contains ~2-fold lower BIAS koffs suggesting more promising binders exist in the sorted versus unsorted population of phage.

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