Fig. 8: CDK4 and CDK6 is highly expressed in breast cancers and ISGs were upregulated in patients treated with palbociclib.

a Expression of CDK4 and CDK6 determined by immunohistochemical in cancerous and benign tissue of breast cancers. b The expression of CDK4 (b) and CDK6 (c) detected by tissue microarray immunohistochemical in cancerous and benign breast cancer tissues (n = 125). d Heat map of mRNA expression of “Interferon stimulated genes” in PBMC of breast cancer patients (n = 5) with or without palbociclib treatment. e Proposed CDK4 and CDK6 blockade induced anti-tumor pathway schematic. Data are presented as the mean ± SD. **p < 0.01. Statistical significance was determined by the Mann–Whitney U test.