Fig. 5: MafB does not participate in the NLRP1, NLRC4, or AIM2 inflammasome activation.
From: MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages
a, b Primed MafB+/+ and MafB−/− PMs were stimulated with the mix of LipoJet liposome and the AIM2 activator poly(dA:dT) (2 µg/ml), the NLRC4 activator Flagellin (1 µg/ml), or the NLRP1 activator muramyl dipeptide (MDP) (10 µg/ml). Levels of supernatant IL-1β were determined by ELISA. Levels of the indicated proteins in the supernatants and cells were determined by western blotting. c Primed MafB+/+ and MafB−/− PMs were treated as in (a, b). Insoluble extracts were cross-linked and ASC oligomerization determined. ASC in the soluble extracts was used as loading controls. d, e Primed MafB+/+ and MafB−/− BMDMs were stimulated with poly(dA:dT) or Flagellin. Supernatant IL-1β was determined by ELISA and the indicated proteins in the supernatants and cells were determined by western blotting. Mean ± SD. Representative of three independent experiments.
