Fig. 7: MafB sustains p62 expression in macrophages. | Communications Biology

Fig. 7: MafB sustains p62 expression in macrophages.

From: MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages

Fig. 7: MafB sustains p62 expression in macrophages.The alternative text for this image may have been generated using AI.

a J774A.1 macrophages were transfected with 50 nM control siRNAs or MafB siRNAs. 48 h after transfection, cells were collected, and protein levels of the indicated genes were determined by western blotting. b Levels of the indicated proteins in control siRNAs or MafB siRNAs transfected macrophages treated with LPS for 4 h, followed by Nigericin stimulation. c Experiment was done as in (a) and levels of SQSTM1 (p62) transcription was determined by real-time PCR. d, e Levels of the indicated proteins in control siRNAs or p62 siRNAs transfected macrophages treated with or without LPS for 4 h (d) and the levels of supernatant TNF-α and IL-6 (e). f, g Levels of supernatant IL-1β (f) and the indicated proteins (g) in control siRNAs or p62 siRNAs transfected macrophages treated with LPS for 4 h, followed by Nigericin or ATP stimulation. Mean ± SD; ***P < 0.001. Representative of three independent experiments.

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