Fig. 9: Mye MafB −/− mice demonstrate elevated LPS and P. aeruginosa induced systemic and pulmonary IL-1β production and deficient lung P. aeruginosa clearance in vivo.
From: MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages
a–c Ten-week MafB fl/fl and Mye MafB−/− mice were injected i.p. saline or LPS (10 mg/kg). Three hours after injection, mice were sacrificed and sera and lung extracts prepared. Levels of the indicated cytokines were determined by ELISA (a, b). Levels of the indicated protein in the lung extracts were determined by western blotting (c). d–f Ten-week MafB fl/fl and Mye MafB−/− mice were i.t. instilled with 0.6 × 107 PAK. 24 h after infection, lung extracts were prepared and levels of the indicated cytokines were determined by ELISA (d); BAL protein levels were measured (e); and lung extracts were diluted and plated. Bacterial colonies were counted to determine CFU (f). g MafB+/+ and MafB−/− BMDMs in 96-well plates were incubated with 2 × 106 PAK for 1 h. The cells were lysed in 100 µl sterile H2O for 20 min and combined with supernatants for serial dilution and plating. Bacterial colonies were counted to determine CFU. n = 3–8; mean ± SD or SE; *P < 0.05, **P < 0.01, ***P < 0.001. PAK P. aeruginosa strain K, CFU colony-forming unit.
