Fig. 1: Actin dynamics and pre- and postsynaptic protein apposition are mis-regulated in dnlg2 mutant NMJs.
From: Neuroligin 2 governs synaptic morphology and function through RACK1-cofilin signaling in Drosophila
a F-actin is downregulated in dnlg2 and cofilin mutants. Confocal images of WT (n = 19), dnlg2 mutants (dnlg2KO70/KO70) (n = 15), and cofilin mutants (tsrN96A/+) (n = 14) third instar larvae NMJ type Ib boutons at muscles 12/13 labeled with Texas Red phalloidin (red, F-actin) and anti-HRP (blue). b Scatter diagram shows a significant decrease in the relative intensity of F-actin in dnlg2 mutants, and cofilin mutants compared with WT. c G-actin is upregulated in dnlg2 and cofilin mutants. Confocal images of WT (n = 29), dnlg1 (dnlg1ex1.9/ex2.3) mutants (n = 23), dnlg2 mutants (n = 30), and cofilin mutants (n = 9) third instar larvae NMJ type Ib boutons at muscles 12/13 labeled with anti-DNase I (green, G-actin) and anti-HRP (blue). d Scatter diagram shows a significant increase in the relative intensity of G-actin in dnlg1 mutants, dnlg2 mutants, and cofilin mutants compared with WT. e Relative apposed active zones decrease in dnlg2 and cofilin mutants. Confocal images of WT (n = 10), dnlg1 mutants (n = 10), dnlg2 mutants (n = 10) and cofilin mutants (n = 8), third instar larvae NMJ type Ib boutons at muscle 4 labeled with anti-GluRIIB (red) and anti-nc82 (green, BRP). White arrowheads highlight the zones that GluRIIB cannot correspond to BRP. f Scatter diagram shows a significant decrease in the relative apposed active zone in dnlg1 mutants, dnlg2 mutants, and cofilin mutants compared with WT. Data are presented as mean ± SEM.
