Fig. 2: Doxo and Abe induce V-ATPase functional suppression in senescent breast cancer cells.
a Four-ellipse Venn diagram outlining overlapped downregulated genes in various therapy-challenged breast cancer cells. b Gene Ontology (GO) enrichment analysis of overlapped downregulated genes (n = 29) shown with biological process (BP), cellular component (CC), and molecular function (MF) terms. All represented GO terms showed significant enrichment at an FDR < 0.05. The color represents the statistical significance of the term. The circle size indicates the counts of enriched genes. c, d Representative fluorescent images of breast cancer cells treated with Bafilomycin A1 (Baf A1; 200 nM) for 3 h stained with pHrodo Green AM (c) and quantitative analysis of the intracellular pH (pHi) (d). e, f Representative fluorescent images and quantitative analysis of lysosomal pH (pHL) in Baf A1 (200 nM)-treated MDA-MB-231 cells stained with LysoSensor Yellow/Blue-DND-160. g, h Representative fluorescent images and quantitative analysis of pHL in Baf A1 (200 nM)-treated MCF-7 cells. Emission (Em) 535 nm (yellow); Em 440 nm (blue). Scale bars represent 50 μm. Data are shown as the means ± SD of three independent experiments. Data were analyzed by unpaired two-tailed Student’s t test (d, f, h) and Fisher’s exact test with Benjamini–Hochberg multiple-testing correction (b). ****P < 0.0001.
