Fig. 2: DMF diminishes incorporation of glutamine into the TCA cycle in HMECs. | Communications Biology

Fig. 2: DMF diminishes incorporation of glutamine into the TCA cycle in HMECs.

From: New insights in the targets of action of dimethyl fumarate in endothelial cells: effects on energetic metabolism and serine synthesis in vitro and in vivo

Fig. 2: DMF diminishes incorporation of glutamine into the TCA cycle in HMECs.The alternative text for this image may have been generated using AI.

A Intracellular aspartate levels in cells treated with 100 μM DMF for 24 h. B Scheme of TCA cycle illustrating labeling from [U-13C]-glutamine. C Fractional labeling of aspartate, (D) fumarate, (E) malate and (F) citrate from [U-13C]-glutamine in cells treated with 100 μM DMF for 24 h. G Intracellular fumarate, (H) malate and (I) citrate levels in cells treated with 100 μM DMF for 24 h. Data are expressed as means ± SD for an experiment with three replicates. Statistical significance was determined using the unpaired, two-sided Student t test and the resulting p values are shown in the figure as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 versus untreated control.

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