Fig. 7: DMF alters metabolism of endothelial cells from caudal fin of zebrafish. | Communications Biology

Fig. 7: DMF alters metabolism of endothelial cells from caudal fin of zebrafish.

From: New insights in the targets of action of dimethyl fumarate in endothelial cells: effects on energetic metabolism and serine synthesis in vitro and in vivo

Fig. 7: DMF alters metabolism of endothelial cells from caudal fin of zebrafish.The alternative text for this image may have been generated using AI.

A Schematics for the experimental conditions used in the fin regeneration assay. B Quantitative analysis of 1H HR-MAS NMR spectra from intact zebrafish fin using Chenomx software. The four experimental conditions shown in the figure are (i) non-regeneration, (ii) regeneration condition treated with the vehicle, (iii) and (iv) regeneration condition treated with 25 µM and 50 µM of DMF, respectively. For the sake of clarity, only relevant metabolite fittings are shown: glucose (blue), glutamine (red), glycine (green), lactate (yellow), serine (purple) and succinate (pink). C Relative abundance compared with the non-regenerated condition for glucose, lactate, glutamine, serine and glycine metabolite. Data are the sum of a pool of sections of the caudal fin from 20 specimens, independently of their biological sex. The amount of material for each experimental condition was as follows: 15.2 mg for non-regenerated fish, 17.6 mg for vehicle condition, 17.8 mg for 25 µM DMF and 15.2 mg for 50 µM DMF.

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