Fig. 1: JNJ-8003 is a picomolar NNI of RSV L + P. | Communications Biology

Fig. 1: JNJ-8003 is a picomolar NNI of RSV L + P.

From: Structural and mechanistic insights into the inhibition of respiratory syncytial virus polymerase by a non-nucleoside inhibitor

Fig. 1

a Domain architecture of RSV L + P. Regions protected by JNJ-8003 binding in an HDX assay and resistance mutations are indicated on top and bottom of RSV L architecture, respectively. CD Connector domain. MT, Cap methyltransferase. NTD N-terminal domain. OD Oligomerization domain. CTD C-terminal domain. b Chemical structure and selected inhibition data of JNJ-8003 (Supplementary Table 1, 2). c Inhibition of RNA synthesis activity of the recombinant RSV L + P by JNJ-8003 in a biotinylated primer extension Flashplate assay. Data points from four technical replicates are shown. d Sequencing gel showing incorporation of a single nucleotide (lane 1 and 4) and multiple nucleotides (lane 2, 3, 5, 6, and 7) to a 4-mer RNA primer. 5 µM JNJ-8003 was used in the assay. ALS-8112-TP is a CTP analog that terminates elongation after its incorporation71. Data points and Gel image are in Supplementary Data 1, 2.

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