Fig. 2: JNJ-8003 inhibits RNA synthesis at early elongation steps. | Communications Biology

Fig. 2: JNJ-8003 inhibits RNA synthesis at early elongation steps.

From: Structural and mechanistic insights into the inhibition of respiratory syncytial virus polymerase by a non-nucleoside inhibitor

Fig. 2: JNJ-8003 inhibits RNA synthesis at early elongation steps.

a Polyacrylamide gel showing single nucleotide incorporation (SNI) from a set of short primers with or without 5 µM JNJ-8003 with le−11 template (+1 site RNA synthesis). b Sequencing gel showing primer extension from a set of short primers with or without 5 µM JNJ-8003 with the le−11 RNA template (+3 site RNA synthesis). c Inhibition of SNI by JNJ-8003 at 5 µM to a set of primers with le−11 RNA template (+1 site RNA synthesis). Bars indicate the mean, and the data points from two independent experiments are shown. The gels are shown in Supplemental Data 1. d Inhibition of SNI by JNJ-8003 at 5 µM to a set of primers with tr−14 RNA template (+1 site RNA synthesis). e Inhibition of SNI by JNJ-8003 at 5 µM to a set of primers with le−11 RNA template (+3 site RNA synthesis). f–h Inhibition curves of JNJ-8003 on SNI with le−11 (+1 site), tr−14 (+1 site), and le−11 (+3 site synthesis), respectively. Data points from two independent experiments and fitted curves are shown. Gel images are in Supplementary Fig. 3 and Supplementary Data 3–10.

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