Table 1 Data collection, reconstruction, and model refinement statistics of cryo-EM structures.
RSV L + P JNJ-8003EMD-29452, PDB: 8FU3 | |
|---|---|
Data collection | |
Microscope | Titan Krios |
Voltage (keV) | 300 |
Nominal magnification | 105000x |
Exposure navigation | Image Shift |
Electron exposure (e /Å2) | 52.66 |
Total exposure time (sec) | 1.5 |
Detector | K3 Summit |
Pixel size (Å)* | 0.825 |
Defocus range (µm) | −1.4 to −2.3 |
Micrographs Used | 11,631 |
Final Refined particles (no.) | 519,118 |
Reconstruction | |
Symmetry imposed | C1 |
Resolution (global) | |
FSC 0.143 | 2.88 Å |
Applied B-factor (Å2) | −127 |
Refinement | |
Protein residues | 1657 |
Ligand | 1 |
Map Correlation Coefficient (Main chain) | 0.73 |
R.m.s deviations | |
Bond lengths (Å) | 0.014 |
Bond angles (°) | 1.01 |
Ramachandran | |
Outliers | 0.54% |
Allowed | 1.57% |
Favored | 97.89% |
Rotamer outliers | 0.07% |
MolProbity score | 0.93 |
EMRinger score | 4.22 |
Clashscore (all atoms) | 1.57 |
