Fig. 5: Primary human cardiac fibroblasts secrete cytokines, chemokines, immunomodulatory proteins in response to IL-1β. | Communications Biology

Fig. 5: Primary human cardiac fibroblasts secrete cytokines, chemokines, immunomodulatory proteins in response to IL-1β.

From: IL-1β-mediated adaptive reprogramming of endogenous human cardiac fibroblasts to cells with immune features during fibrotic remodeling

Fig. 5: Primary human cardiac fibroblasts secrete cytokines, chemokines, immunomodulatory proteins in response to IL-1β.

a Chemokine CCR2 and cytokine IL-1R gene expression performed in adult rat ventricular cardiac fibroblasts treated with Veh or IL-1β (10 ng/mL) after 24 h using quantitative PCR (qPCR). n = 3, *P < 0.05. b IL-8 and pro-differentiation TGFβ gene expression levels in primary hVCF treated with Veh or IL-1β (10 ng/mL) after 24 h using quantitative PCR (qPCR). n = 3, *P < 0.05. c Heatmap representation of secreted cytokines and chemokines (IL6, IL8, MCP-1, MCP-3, IL12p70, IL17A, IL10, 1L1a, IL1β, IL3, TNFa, VEGF, MIP1b, MIP1a and IL1RA) in the conditioned media after 24 h of treatment with Veh or IL-1β (10 ng/mL) profiled using a milliplex bead-based detection method. The values in the heatmap are expressed in Raw Z-score, ranging from blue (-2) to orange (+2). d Scatterplots of cytokines and chemokines (IL-6, IL-8, IL-10 and MCP-3) quantified in the condition media from Veh and IL-1β treated cardiac fibroblasts. The scatterplots represent Mean ± SD values (n = 3 biological replicates, *P < 0.05) by unpaired-student t-test with Mann–Whitney post hoc test. e GO Function analysis to identify the function of the 158 secretory proteins unique to conditioned media from IL-1 β treated cells using LC-MS/MS. Benjamini–Hochberg score of false discovery rate.

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