Fig. 3: Time course of the autoprocessing of ⁽⁻¹⁰²⁾MPro^M and ⁽⁻²⁵⁾MPro^1–199 as a function of decreasing protein concentration.

a, b Precursor constructs showing the products released upon N-terminal autoprocessing. Time course of (c) ⁽⁻¹⁰²⁾MPro^M and (d) ⁽⁻²⁵⁾MPro^1–199 autoprocessing. Reactions were initiated at the indicated concentration in buffer B at 28 °C (see Tables S1, S2 for details). Aliquots of the reaction were drawn at the indicated times and terminated by the addition of gel sample buffer. Samples (2.6–3.6 µg/lane) were subjected to SDS-PAGE and visualized by staining. Precursor, reaction products, and molecular weight standards (M) are indicated in kDa.