Table 2 Catalytic activity, dimer dissociation and GC373 binding constants of mature MPro and its precursor analogues.

 MPro^{WT b}

0.6 ± 0.05

1.3 ± 0.2

0.15 ± 03 ^c

Dimer

 MPro^R298A

0.31 ± 0.01 ^d

0.0435 ± 0.002

7.13 ± 0.40

0.2 ± 0.04 ^c

Dimer

 MPro^E290A

(2.82 ± 0.04) 10^{−3 d}

(8.0 ± 0.40) 10⁻⁶

353 ± 21

3.5 ± 0.5 ^c

4.8 ± 0.6 ^e

Dimer

 MPro^{M b}

(3.4 ± 0.13) 10⁻³

(5.0 ± 0.15) 10⁻⁷

6600

6.13 ± 0.3 ^c

6.7 ± 0.2 ^e

Dimer

 MPro^{1–199 b}

(1.15 ± 0.03) 10⁻⁶

32 ± 5 ^c

Monomer

 ⁻¹⁰²MPro^M

19.2 ± 4.4 ^c

Monomer

 MPro^M-IP

(6.04 ± 0.15) 10⁻⁶

Monomer

(9.40 ± 0.90) 10^{−4 d}

6.02 ± 1.2 ^c

8.5 ± 1.5 ^e

Dimer