Table 1 Comparisons between neonatal niche injection and blastocyst complementation, as well as fetal injection.

From: Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity

 

Neonatal niche injection (P1.5)

Blastocyst complementation (E2.5)9

Fetal injection (Exo utero, E13.5)11

Second surgery

Not required

Embryo transfer to pseudopregnant mice

Cesarean section and transfer to foster mothers

Host survival rate

85% (33 of 39)a

43% (34 of 79)b p < 0.0001c

74% (14 of 19)d p = 0.47c

Percentage of donor nephron engraftment in kidneys relative to surviving hosts

100% (33 of 33)

71% (24 of 34)e p < 0.0001c

14% (2 of 14) p < 0.0001c

Percentage of donor nephron engraftment in kidneys relative to all injected hosts

85% (33 of 39)

30% (24 of 79) p < 0.0001c

11% (2 of 19) p < 0.0001c

  1. aHosts that survived 2 weeks after the surgery performed by the skilled experimenter.
  2. bPercentage of pups that reached adulthood from embryos derived from intercrosses of Sall1+/− mice injected with embryonic stem cells.
  3. cComparisons against neonatal niche injection using the Chi-square test.
  4. dPercentage of fetuses that survived until cesarean section performed by the skilled experimenter. Note the subsequent risk of being killed by foster mothers.
  5. ePercentage of adults derived from embryos injected with embryonic stem cells, exhibiting confirmed chimera formation.
Back to article page