Fig. 2: Efficient proliferation of MuSC requires TA³.

a Schematic illustration of tamoxifen injection regime followed by ex vivo culture of myofibers for the times indicated, 72 h shown here. Genotypes of mutant and control mice are shown. b Clusters of MuSC were counted and plotted as the total number of nuclei per fibre or the number of clusters per fibre. Fewer MuSC-derived cells were present in TA^3iSC-KO compared to controls. c Pax7 and MyoD or e MyoD and MyoG immunostaining of MuSC progeny on myofibers isolated from control or TA^3iSC-KO mice (scale bar = 50 μm). d Graphs show the total number of nuclei per myofiber in control and TA^3iSC-KO fibres, or the percentage of Pax7⁺ (red), MyoD⁺ (green) or Pax7⁺/MyoD⁺ (blue) double positive cells *p < 0.05, n = 9, unpaired, two-tailed t test. f Graphs show the total number of nuclei per myofiber in control and TA^3iSC-KO fibres, or the percentage of MyoG⁺ (red), MyoD⁺ (green) or MyoG⁺/MyoD⁺ (blue) double positive cells *p < 0.05; **p < 0.01, n = 9, unpaired, two-tailed t test, mean and SD are shown.