Fig. 5: Control of fibroblast phenotype (FB vs. myoFB) with SD208 treatment. | Communications Biology

Fig. 5: Control of fibroblast phenotype (FB vs. myoFB) with SD208 treatment.

From: Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts

Fig. 5

a Immunocytochemistry images of FB-CM co-cultures with and without SD-208 treatment, labeled for vimentin (red), alpha-SMA (green) and nuclei by DAPI (blue). b Quantification of alpha-SMA positive FBs based on fluorescence with time in culture. The SD208 treatment group showed significantly less α-SMA expression compared to untreated, with the difference increasing over time, *p < 0.01; **p < 0.001. c, d Western blot and quantification of the level of α-SMA protein, normalized by GAPDH. SD208 treatment significantly reduced the level of α-SMA in fibroblasts (switch of myoFB to FB); ChR2-transformation further reduced α-SMA levels, *p < 0.1; **p < 0.05.

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