Fig. 1: KV2.1 macro-clusters are declustered into micro-clusters with the KV2.1S586A point mutation. | Communications Biology

Fig. 1: KV2.1 macro-clusters are declustered into micro-clusters with the KV2.1S586A point mutation.

From: The formation of KV2.1 macro-clusters is required for sex-specific differences in L-type CaV1.2 clustering and function in arterial myocytes

Fig. 1

a Confocal maximum intensity projection images of HEK293T cells transfected with KV2.1WT or KV2.1S586A tagged with mScarlet. Insets show single plane images of the bottom or center of each cell. b Number of KV2.1 cluster volumes of KV2.1WT (black) and KV2.1S586A (blue) in transfected HEK293T cells. Insets quantify relative frequency histograms as a percentage of KV2.1 volumes. Plots representing (c) number of clusters per cell, (d) total cluster volume per cell, and (e) mean cluster volumes (n = 11 KV2.1WT and 11 KV2.1S586A cells). f Representative super-resolution GSD images of immunolabeled KV2.1 channels in transfected HEK293T cells. Insets show 4 μm2 regions of interest. Red overlay depicts Gaussian blur. Cyan lines indicate distances of greater than 200 nm while yellow arrows represent distances less than 200 nm. g Number of clusters of KV2.1WT (black) and KV2.1S586A (blue) by cluster area in transfected HEK293T cells. Insets quantify relative frequency histograms as a percentage of KV2.1 areas. h Summary plot of mean KV2.1 cluster areas from super-resolution images (n = 9 KV2.1WT and 8 KV2.1S586A cells). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SEM.

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