Fig. 2: Penk-deficient chimeric mice exhibit alteration in the relative frequency of mucosal immune cells. | Communications Biology

Fig. 2: Penk-deficient chimeric mice exhibit alteration in the relative frequency of mucosal immune cells.

From: Proenkephalin deletion in hematopoietic cells induces intestinal barrier failure resulting in clinical feature similarities with irritable bowel syndrome in mice

Fig. 2

Cytofluorometric analysis of the relative frequency of innate and adaptive immune cells in colon mucosa of penk+/+ (white circles and white histogram) and penk−/− (black circles and gray histogram) chimeric mice (n = 11). a Deep phenotyping of CD45+ cells performed using Phenograph for clustering and UMAP for dimensionality reduction. Phenograph clusters are annotated according to characteristic marker expression (Supplementary Fig. 4). Nearest-neighbors clusters were unsupervised delineated using phenograph plug-in. b Frequencies of mucosal immune cell subsets identified by this method. Each symbol represents one mouse. Statistical analysis was performed using Mann-Whitney U test. * p < 0.05; ** p < 0.01.

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