Fig. 5: Expression levels of monoamine-regulating enzymes in the brain of SMNΔ7 mice.
From: SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy
a, h, o Representative autoradiograms of brain lysates immunoblots of SMNΔ7 and wild-type (WT) mice at a post-natal day 3, h day 6, and o day 11. b–g Protein levels quantification of b Tyrosine hydroxylase (TH), c phospho-Tyrosine hydroxylase at Ser-40 (P-Ser40-TH), d Dopamine β hydroxylase (DβH), e Tryptophan hydroxylase 2 (TPH2), f Aromatic amino acid decarboxylase (AADC) and g Monoamine Oxidase A (MAO-A) in SMNΔ7 (n = 4) and WT (n = 5) mice (except for TPH2: n = 4 mice/genotype) at post-natal day 3. i–n Protein levels quantification of i TH (n = 4 WT, n = 5 SMNΔ7), j P-Ser40-TH (n = 4 WT, n = 5 SMNΔ7), k DβH (n = 4 WT, n = 4 SMNΔ7), l TPH2 (n = 4 WT, n = 3 SMNΔ7), m AADC (n = 4 WT, n = 5 SMNΔ7) and n MAO-A (n = 4 WT, n = 5 SMNΔ7) at post-natal day 6. p–u Protein levels quantification of p TH (n = 13 WT, n = 14 SMNΔ7), q P-Ser40-TH (n = 4 WT, n = 5 SMNΔ7), r DβH (n = 4 WT, n = 5 SMNΔ7), s TPH2 (n = 4 WT, n = 5 SMNΔ7), t AADC (n = 13 WT, n = 14 SMNΔ7) and u MAO-A (n = 8 WT, n = 10 SMNΔ7) at post-natal day 11. Data are normalized to β-Actin levels and shown as box and whisker plots representing the median with interquartile range (IQR). Dots represent individual mice values. *p < 0.05, **p < 0.01, compared with age-matched WT mice (unpaired t-test).
